FIGURE 6.

Comparison of the sensitivity of the different cellular biosensors in detecting tau seeded aggregation. (a) Treatment of an increasing concentration of tauRD fibrils to human embryonic kidney 293 (HEK293) cells expressing tauRD‐Nluc/Cluc induces aggregation of the split luciferase complementation (SLC) biosensor as shown by an increase in the luciferase luminescence.⁹⁴ (b) Treatment of an increasing concentration of tauRD fibrils to HEK293 cells expressing tauRD(ΔK)‐CFP/YFP induces aggregation of the fluorescence resonance energy transfer (FRET) biosensor. The ΔK indicates ΔK280 variant of tau.⁶⁶ (c) Dose‐dependent increase in tau‐bimolecular fluorescence complementation (BiFC) fluorescence induced with tau K18‐P301L proteins in Venus‐based tau‐BiFC fluorescence turn‐on biosensor expressed in HEK293 cells.⁹⁹ Permissions obtained from References 94, 66, and 99