Changes in gene expression induced by organic extracts (5 h exposure, 5 μg mL−1).
| Gene | Relative gene transcription levelsa,c | ||||||
|---|---|---|---|---|---|---|---|
| Control | Beijing summer | Beijing winter | Shanxi summer | Shanxi winter | Sichuan summer | Sichuan winter | |
| Cat | 1 ± 0.05 (a) | 1.1 ± 0.2 (a) | 1.1 ± 0.1 (a) | 1 ± 0.3 (a) | 1.2 ± 0.2 (a) | 1 ± 0.1 (a) | 1.4 ± 0.2 (a) |
| cyp1a1 b | 1 ± 0.02 (a) | 1.8 ± 0.2 (b) | 1.5 ± 0.1 (ab) | 1.6 ± 0.1 (b) | 1.5 ± 0.1 (b) | 1.6 ± 0.1 (b) | 1.7 ± 0.1 (b) |
| cyp1b1 | 1 ± 0.05 | 1.3 ± 0.1 | 1.3 ± 0.1 | 1.4 ± 0.2 | 1.5 ± 0.1 | 1.3 ± 0.2 | 1.3 ± 0.1 |
| HO-1 | 1 ± 0.1 | 1.8 ± 0.5 | 1.4 ± 0.2 | 1.6 ± 0.2 | 1.5 ± 0.3 | 1.1 ± 0.2 | 1.5 ± 0.5 |
| IL-6 b | 1 ± 0.1 (a) | 4 ± 0.6 (b) | 4.5 ± 1 (b) | 2.7 ± 0.5 (ab) | 4.2 ± 0.8 (b) | 3.6 ± 0.6 (ab) | 3.8 ± 0.8 (ab) |
| IL-8 b | 1 ± 0.03 (a) | 4.5 ± 0.8 (bcd) | 6.6 ± 0.6 (d) | 3.2 ± 0.6 (b) | 5.5 ± 0.5 (cd) | 4 ± 0.2 (bc) | 4.9 ± 0.4 (bcd) |
| NQO-1 | 1 ± 0.04 | 0.9 ± 0.1 | 0.9 ± 0.04 | 0.9 ± 0.1 | 0.9 ± 0.05 | 0.9 ± 0.04 | 0.9 ± 0.03 |
| SOD1 b | 1 ± 0.03 (a) | 1.4 ± 0.1 (b) | 2.1 ± 0.1 (c) | 1.3 ± 0.1 (ab) | 1.1 ± 0.1 (ab) | 1.3 ± 0.1 (ab) | 1.2 ± 0.1 (ab) |
| SOD2 b | 1 ± 0.01 (a) | 0.8 ± 0.1 (b) | 0.8 ± 0 (ab) | 0.9 ± 0.1 (ab) | 0.8 ± 0 (b) | 0.8 ± 0 (b) | 0.9 ± 0.1 (ab) |
a
Data are normalized to the control for each gene and presented as average ± SEM.
b
Bolded gene: statistically significant ANOVA (p < 0.05).
c
Means with different letters are significantly different at p < 0.05 (Tukey HSD test; shown only for genes with statistically significant ANOVA).