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. 2021 Sep 9;9:714601. doi: 10.3389/fcell.2021.714601

FIGURE 4.

FIGURE 4

hsa_circ_0000073 targets miR-1252-5p to promote OS progress. (A) qRT-PCR examined expression of four candidate miRNAs after hsa_circ_0000073 silencing. (B) qRT-PCR measured expression of miR-1252-5p in MG-63 and Saos-2 cells after overexpression or knockdown of hsa_circ_0000073. (C) qRT-PCR confirmed lower expression level of miR-1252-5p in OS cells. (D) Luciferase reporter assay confirmed relationship between hsa_circ_0000073 and miR-1252-5p in HEK293 cells. (E,F) CCK-8 and clone formation assays showed miR-1252-5p inhibited cell proliferation in OS cells. (G,H) Wound-healing and transwell assays revealed miR-1252-5p restrained migration and invasion ability in OS cells with regulation, magnification, ×200. (I) Flow cytometry analysis indicated upregulation of miR-1252-5p induced S phase arrested in OS cells whereas downregulation of reductions. Data were presented as mean ± SD. *P < 0.05, **P < 0.005, ***P < 0.001.