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. 2021 Sep 9;9:714601. doi: 10.3389/fcell.2021.714601

FIGURE 5.

FIGURE 5

hsa_circ_0000073 regulated expression of CCNE2 and MDM2 through miR-1252-5p. (A) qRT-PCR determined expression of five candidate genes after hsa_circ_0000073 silencing. (B,C) qRT-PCR and WB assays assessed expression of CCNE2 and MDM2 in OS cells with hsa_circ_0000073 regulation. (D,E) qRT-PCR and WB assays confirmed expression level of CCNE2 and MDM2 was higher in OS cells. (F) WB verified impacts of miR-1252-5p on CCNE2 and MDM2 expression in OS cells. (G,H) Luciferase reporter assay and RIP assays confirmed relationship among hsa_circ_0000073, miR-1252-5p, CCNE2, and MDM2 in HEK293 cells. (I,J) WB revealed rescue ability of miR-1252-5p, CCNE2, and MDM2 on overexpressed-hsa_circ_0000073 in MG-63 and Saos-2 cells. Data were presented as mean ± SD. *P < 0.05, **P < 0.005, and ***P < 0.001.