FIG 3.

Fatty acid profiles of E. faecalis WT, ΔFASII, and Δcfa strains grown without and with serum. E. faecalis was grown in RPMI+ or in RPMI+ containing 40% human serum to the stationary phase. FA composition was compared to assess the roles of medium FA content and genetic background. The means of the percentages of each FA found in E. faecalis BWT or mutant strains grown to the stationary phase in the presence or absence of 40% serum of three independent experiments are presented (see also Tables S2 to S4 in the supplemental material). (A) BWT-FASII grown in the absence or presence of serum. (B). BWT-FASII and EfΔFASII strains grown in 40% serum. (C) BWT-cfa and EfΔcfa strains grown in RPMI+. (D) BWT-cfa and EfΔcfa strains grown in 40% human serum. Only major fatty acids (>2% of total in at least one profile) are shown. Stars, fatty acids derived from serum. Green arrows, cyclopropanation in the BWT strains of unsaturated fatty acids; dark green, produced by E. faecalis (mainly 18:1Δ11); light green, incorporated from serum (mainly 18:1Δ9). Columns are colored as follows: dark green, BWT-FASII; dark blue, EfΔFASII; light green, BWT-cfa; light blue, EfΔcfa; hatched, strains grown in RPMI+; empty, strains in RPMI+ supplemented with 40% human serum.