FIG 1.

OsaR binds to the intergenic region upstream of fleQ in vivo and in vitro. (A) OsaR immunoprecipitation reads (in red) were plotted against the number of reads from the nonimmunoprecipitated DNA (in blue). The ordinate indicates the coverage depth of the reads at a certain genomic location. The region represented by the abscissa corresponds to the genomic location from coordinates 1187000 to 1193000. The locations of the fleQ and fleSR genes are represented in boxes below the abscissa. Peaks (reads, >1,500) represented by DNA accumulations are marked with dashed lines. (B) Gel shift assays of OsaR and the intergenic DNA upstream of fleQ or the DNA fragments covering both the intergenic region and coding region of fleS (fleS1 to fleS4). The protein-DNA complex is indicated by an arrowhead. The length and location of the fragments are shown in panel A; red and black colors indicate shift or no shift, respectively. Purified OsaR was used in a concentration gradient of 0, 20 nM, and 40 nM for fleQ or 0 (−) and 40 nM (+) for fleS1 to fleS4; DNA fragments of 100 ng were used.