FIG 5.
fleQ and swimming motility are upregulated when osaR and vfr are both deleted. (A) The effect on fleQ transcription by deleting osaR and vfr simultaneously. The mRNA levels were determined by RT-qPCR. (B) The effect on the promoter activity of fleQ by deleting both osaR and vfr. Strains were transformed with the reporter plasmid PfleQ-lacZ, followed by β-galactosidase assays when cultured to an OD600 of ∼0.6. (C) The effect on swimming motility by deleting both osaR and vfr. Motility was detected and quantitated by three independent replicates. ns, not significant; *, P < 0.05; ***, P < 0.001; determined by Student's t test. Error bars represent standard deviations.