TABLE 2.
Primers used in this study
| Oligonucleotide | Sequence (5′–3′)b | Purpose |
|---|---|---|
| PfleQ-Fa | CCGGAATTCGTTTTTCATGGCTTTGTGCCG | Construction of PfleQ-lacZ |
| PfleQ-R | CGCGGATCCTTTGATCAGCTGCCTTGCATC | Construction of PfleQ-lacZ |
| pKF912-F | CCCAAGCTTGAAGGCTTCGCAGCTCTC | Construction of pKF912 |
| pKF912-R | GGAATTCCTGAAGCGCCTGTTCTTCC | Construction of pKF912 |
| Δvfr-F | CGATGATGTCGACAACATCG | Identification of vfr deletion |
| Δvfr-R | TCAAGAGCCAGACCCTGATG | Identification of vfr deletion |
| fleS1-F | GGCATGAGCCGGCGTGAC | Amplification of fleS1 |
| fleS1-R | CGTATCGGCTGGCTGCTC | Amplification of fleS1 |
| fleS2-F | ATGCAACCAGCCCTCAACGC | Amplification of fleS2 |
| fleS2-R | TGACGATGACGCCGCCGG | Amplification of fleS2 |
| fleS3-F | GCGGAAAAGGAGCGCCTGG | Amplification of fleS3 |
| fleS3-R | GTTGAGCAGGATCAGTTGCC | Amplification of fleS3 |
| fleS4-F | AGCCGTTGGTGGGCATGC | Amplification of fleS4 |
| fleS4-R | CGGGCGAACACCAGCATG | Amplification of fleS4 |
| fleQF123-F | GTTTTTCATGGCTTTGTGCC | Amplification of F1, F2, F3 |
| fleQF1456-R | TTTGATCAGCTGCCTTGCATC | Amplification of F1, F4, F5, F6 |
| fleQF2-R | AGCCAGCATTTGGCCACTA | |
| fleQF3-R | CAAATGTGAACGATTAGTCA | Amplification of F2 |
| fleQF4-F | ACTTAACTAGTGGCCAAATGC | Amplification of F3 |
| fleQF5-F | TAAAATTGACTAATCGTTCAC | Amplification of F4 |
| fleQF6-F | GCAAAAAAAACACGCCATAA | Amplification of F5 |
| fleQF7-F | GCAAAAAAAACACGCCATAAAATTGACTAATCGTTCACATTTGACTTAACTAGTGGCCAAATGCTGGCT | Amplification of F6 |
| fleQF7-R | AGCCAGCATTTGGCCACTAGTTAAGTCAAATGTGAACGATTAGTCAATTTTATGGCGTGTTTTTTTTGC | Annealing of F7 |
| fleQF8-F | ACACGCCATAAAATTGACTAATCGTTCACATTTGACTTAACTAGTGGCCA | Annealing of F7 |
| fleQF8-R | TGGCCACTAGTTAAGTCAAATGTGAACGATTAGTCAATTTTATGGCGTGT | Annealing of F8 |
| fleQF7m1-F | GCAAAAAAAACACGCCGCAAAATTGACTAGCCGTTCACATTTGACTTAACTAGTGGCCAAATGCTGGCT | Annealing of F8 |
| fleQF7m1-R | AGCCAGCATTTGGCCACTAGTTAAGTCAAATGTGAACGGCTAGTCAATTTTGCGGCGTGTTTTTTTTGC | Annealing of F7m1 |
| fleQF7m2-F | GCAAAAAAAACACGCCATAAAATTGACTAATCGTTCACGTTTGACTTAACCAGTGGCCAAATGCTGGCT | Annealing of F7m1 |
| fleQF7m2-R | AGCCAGCATTTGGCCACTGGTTAAGTCAAACGTGAACGATTAGTCAATTTTATGGCGTGTTTTTTTTGC | Annealing of F7m2 |
| fleQF7m3-F | GCAAAAAAAACACGCCGCAAAATTGACTAGCCGTTCACGTTTGACTTAACCAGTGGCCAAATGCTGGCT | Annealing of F7m3 |
| fleQF7m3-R | AGCCAGCATTTGGCCACTGGTTAAGTCAAA | Annealing of F7m3 |
| fleQFP-F-FAM | CGTGAACGGCTAGTCAATTTTGCGGCGTGTTTTTTTTGC | DNase I footprinting assay |
| fleQFP-R | TTTATTGCCTCTCCCTGTCG | DNase I footprinting assay |
| fleQ-qRT-F | AGCCAGCATTTGGCCACTA | qRT-PCR |
| fleQ-qRT-R | ACTACCGCCTCAACGTATTCC | qRT-PCR |
| rpsL-qRT-F | CGCTTCTCATGCTCCATCC | qRT-PCR |
| rpsL-qRT-R | ACGTGCCTGCGCTGCAAAACCCCGAGGTGTCCAGCGAACC | qRT-PCR |
a
F, forward; R, reverse; FAM, 6-carboxyfluorescein phosphoramidate labeled at the 5′end.
b
The underlines are the sites of restriction enzymes.