FIG 3.

Interactions between the rod proteins and SltF. Coimmunoprecipitation of the structural rod proteins FliE (A), FlgB (B), FlgC (C), FlgF (D), and FlgG (E) in the presence of wild type SltF and the mutant versions of SltFΔ95, SltFΔ47 and SltFΔ48 was performed as described in Materials and Methods. The assays were performed with anti-FliE (A), anti-FlgB (B), anti-FlgC (C), anti-FlgF (D), and anti-FlgG (E). All purified proteins were used at a concentration of 0.3 μM and loaded onto 17.5% SDS-PAGE gels, and anti-His polyclonal antibodies were used to detect the proteins. Molecular mass markers are shown on the right.