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Lytic activity of SltF tested by turbidimetry. The lysis of an insoluble substrate kept in suspension by constant mixing was recorded over time, measuring the absorbance at 450 nm. The arrow indicates the addition of buffer, SltF, SltF plus FlgJ, and SltF plus FlgB. The indicated proteins present in the reaction are in equimolar concentrations. For further details, see Materials and Methods.
