FIG. 8.

Effector mutation T35S induces a dominant-negative effect in Ras(D119N) as shown by BrdU incorporation in NIH 3T3 cells. Histograms show the effect of transfection of serum-stimulated NIH 3T3 cells by pEXV-KF plasmids expressing Ras(D119N), Ras(T35S/D119N), Ras(E37G/D119N), or Ras(Y40C/D119N). Depicted is the percentage of transfected cells showing DNA synthesis as detected with the BrdU assay. The percentage is the mean of at least two experiments in which 100 to 200 transfected cells were counted. Transfected cells were determined by cotransfection with pEGFP and detection by fluorescence microscopy. The bars indicate the deviation.