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. 2021 Sep 9;9:715717. doi: 10.3389/fbioe.2021.715717

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Copyright © 2021 Alvino, Thomas, Ghorbel, Rapetto, Narayan, Kilcooley, Iacobazzi, Carrabba, Fagnano, Cathery, Avolio, Caputo and Madeddu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ECM remodeling of the implanted artery. (A,B) Tiled images and inserts at higher magnification display the composition of elastic fibers of the LPA and graft, as assessed using EVG (A) and Azan Mallory (B) stainings. (C–E) Bar graphs showing the results of the quantification of elastin and collagen and their ratio. Images were visualized using a light microscope with 2.5x, 10x and 20x objectives. N = 5 biological replicates per experimental group. per experimental group. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001.