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Duplexes formed between the nascent transcript at the N TRS-B and the 5ʹ UTR depending on length of the transcript. The second most abundant sgRNA is formed from a non-canonical duplex with a sequence adjacent to the core sequence (left-hand side). CS-L – proposed leader core sequence in the 5ʹ UTR, cCS-B – Sequence complementary to the core sequence in the body, SL1 – Stem-loop 1, SL2 – Stem-loop 2
