KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-typhoid toxin MAbs and Fabs | This manuscript | See Method Details |
| Chemicals, peptides, and recombinant proteins | ||
| Typhoid Toxin, Toxoid, & PltB subunits | This manuscript | See Method Details |
| Bacterial and virus strains | ||
| S. Typhi ISP2825 | Galán and Curtiss, 1991 | NCBI Genbank accession number CP080960 |
| S. Typhi ISP2825 carrying cdtBH160Q | Spanò et al., 2008 | N/A |
| E. coli Acella strain (E. coli BL21(DE3) ΔendA ΔrecA) | Originally obtained from EdgeBio. Note that the Acella strain is no longer available from the vendor. | Competent cells were prepared in our laboratory. |
| Critical commercial assays | ||
| Alexa Fluor 555 Protein Labeling Kit | ThermoFisher | A20174 |
| RNeasy mini kit | QIAGEN | 74104 |
| iScript cDNA synthesis kit | Bio-Rad Laboratories | 1708891 |
| Herculase II Fusion DNA polymerase | Agilent | 600677 |
| Phusion DNA polymerase | New England BioLabs | M0530 |
| QIAEX DNA Extraction Kit | QIAGEN | 20021 |
| Deposited data | ||
| Typhoid toxin bound to TyTx1 Fab or TyTx4 Fabs | RCSB Protein Data Bank | PDB: 7K7H and PDB: 7K7I (see Table S1) |
| Complete genome sequence of S. Typhi ISP2825 | GenBank | CP080960 |
| Experimental models: Cell lines | ||
| Henle-407 Cells | Originally obtained from ATCC | Validated using intestinal epithelial markers |
| Jurkat Cells | Originally obtained from ATCC | TIB-152 |
| Sp2/0-Ag14 | Originally obtained from ATCC | CRL-1581 |
| Experimental models: Organisms/strains | ||
| BALB/c | Taconic Biosciences | N/A |
| Oligonucleotides | ||
| See Table S3 for oligonucleotide information | IDT | See Table S3 |
| Software and Algorithms | ||
| GraphPad Prism 6 or 8.3 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| LI-COR Image Studio | LI_COR Image Studio Software | https://www.licor.com/bio/image-studio/resources |
| Excel | Microsoft | Office 365 |
| FlowJo V10 | TreeStar | https://www.flowjo.com/ |
| ImageJ | National Institutes of Health | https://imagej.nih.gov/ij/ |
| Unicorn 6.3 | GE Healthcare Life Sciences | https://www.cytivalifesciences.com/en/us/shop/unicorn-6-3-p-01118 |
| BLASTp | National Institutes of Health | https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins |
| Biacore T200 Evaluation Software | GE Healthcare | https://www.cytivalifesciences.com/en/us/shop/protein-analysis/spr-label-free-analysis/systems/biacore-t200-p-05644 |
| CryoSparc 2 | Punjani et al., 2017 | https://cryosparc.com |
| Relion 3 | Zivanov et al., 2018 | https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page |
| PyMol | Schrodinger | https://pymol.org/2/ |
| Chimera | UCSF Chimera | https://www.cgl.ucsf.edu/chimera/ |
| PHENIX | Adams et al., 2010 | https://phenix-online.org/ |
| Coot | Emsley et al., 2010 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |