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. 2021 Sep 13;17(9):e1009900. doi: 10.1371/journal.ppat.1009900

Fig 1. TRIM25 is upregulated upon IBDV infection.

Fig 1

(A) The RNA-seq analysis of host molecules from host defense upon IBDV infection compared to that of non-infected in DF-1 cells at 24 h p.i. (B and C). IBDV infection causes the mRNA levels upregulation of TRIM25 in DF-1 cells. DF-1 cells were mock-infected or infected with IBDV Gt strain at an MOI of 0.1 and analyzed at 12, 24, 36, and 48 h p.i., respectively. (B) Relative fold-change of IBDV genome mRNA of IBDV of cell samples was quantified by RT-qPCR. (C) Relative expression of TRIM25 was quantified by RT-qPCR compared to the control. (D and E) IBDV infection causes upregulation of TRIM25 in DT40 cells. DT40 cells were mock-infected or infected with IBDV Gt strain at the MOI of 5 and analyzed at 12, 24, 36, and 48 h p.i., respectively. (D) The relative fold change of mRNA level of IBDV genome in cell samples were quantified by RT-qPCR. (E) Relative expression levels of TRIM25were quantified by RT-qPCR. All qPCR results are represented as relative fold changes normalized to β-actin controls. Three independent experiments were performed, and data are shown as mean ± standard deviations for triplicates from a representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, no significant difference.