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. 2021 Sep 9;8:719612. doi: 10.3389/fnut.2021.719612

Table 2.

Daily nutrient intake and gastrocnemius muscle weights.

Young Aged
CON CAFE CON CAFE
Energy intake (kcal/kg b.w.) 193 ± 41.5 244 ± 41.4 156 ± 9.7 177 ± 11.2
Total fat (grams/kg b.w.) 2.7 ± 0.6 11.8 ± 1.9*** 2.2 ± 0.14 8.2 ± 0.6**
Saturated fat (grams/kg b.w.) 0.51 ± 0.11 5.1 ± 0.8*** 0.41 ± 0.03 3.4 ± 0.2***, †††
Cholesterol (mg/kg b.w.) 0.08 ± 0.02 13.6 ± 2.6*** 0.06 ± 0.00 8.4 ± 1.1**
Total carbohydrates (grams/kg b.w.) 21.3 ± 4.6 24.5 ± 4.1 17.2 ± 1.0 18.6 ± 1.3
Sugar (grams/kg b.w.) 2.7 ± 0.6 12.7 ± 2.0*** 2.2 ± 0.14 9.7 ± 1.0***
Protein (grams/kg b.w.) 10.9 ± 2.3 7.7 ± 1.4 8.8 ± 0.6 5.3 ± 0.3
Gastrocnemius muscle weight (mg) M: 2022 ± 88 F: 1034 ± 66 M: 1425 ± 130F: 1229 ± 113 M: 1494 ± 137 F: 947 ± 58 M: 1347 ± 275F: 1056 ± 101

N = 4 cages/group. CON, Control diet; CAFÉ, high-fat calorie-dense diet; M, Male; F, Female. Excepted where stated, values expressed per kilogram of body weight and represent means ± SEM.

**

P < 0.01,

***

P < 0.001: significantly different from aged-matched control.

†††

P < 0.001: significantly different from young cafeteria treated animals.

P < 0.05: significant main effect of cafeteria diet.