Table 3.
Mitochondrial respiratory capacity in gastrocnemius permeabilized myofibers in response to experimental cafeteria diet. Determined following 12-week exposure to either a control (CON) or high-fat calorie dense diet (CAFE).
| Mitochondrial respiration (pmol/sec/mg) | Young | Aged | ||
|---|---|---|---|---|
| CON | CAFE | CON | CAFE | |
| Leak—State 2 | 2.85 ± 0.69 | 3.54 ± 0.75 | 2.19 ± 0.28 | 3.10 ± 0.65 |
| Phosphorylating—State 3i | 62.9 ± 12.7 | 64.1 ± 12.7 | 55.8 ± 8.7 | 70.8 ± 7.0 |
| Phosphorylating—State 3i+II | 65.2 ± 14.6 | 67.4 ± 14.0 | 59.3 ± 8.9 | 73.2 ± 7.1 |
| Maximal respiration (electron transfer capacity) | 76.8 ± 17.0 | 82.2 ± 17.4 | 73.1 ± 11.1 | 84.4 ± 6.9 |
Values represent means ± SEM. No significant differences observed between groups. n = 7–8/group.