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. 2021 Sep 17;59(5):86. doi: 10.3892/ijo.2021.5266

Figure 2.

Figure 2

LINC00514 Knockdown suppresses the proliferation, invasion and expression of lipogenesis-related proteins in ESCC cells. (A) Reverse transcription-quantitative PCR assay of LINC00514 expression in various ESCC cell lines, including Eca109, KYSE150, KYSE30, KYSE450 and KYSE70 as well as in the normal esophageal epithelial Het-1A cell line. Data were compared using an ANOVA followed by a post hoc Dunnett's test. *P<0.05, **P<0.01, ***P<0.001 vs. Het-1A. (B) Three siRNAs specific for LINC00514 significantly downregulated the expression of LINC00514 in KYSE150 and KYSE30 cells. Data were compared using an ANOVA followed by a post hoc Dunnett's test. (C-E) LINC00514 siRNA2 suppressed the proliferation and invasion of KYSE150 and KYSE30 cells. (F) LINC00514 siRNA2 reduced the expression of lipogenesis-related proteins (SPHK1, FASN, ACACA and SCD1) in KYSE150 and KYSE30 cells. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. si-NC group. LINC00514, long intergenic nonprotein-coding RNA 00514; ESCC, esophageal squamous cell carcinoma; SPHK1, sphingosine kinase 1; FASN, fatty acid synthetase; ACACA, acetyl-CoA carboxylase α; SCD1, stearoyl-CoA desaturase 1; siRNA, small interfering RNA.