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. 2021 Sep 22;41(38):7965–7977. doi: 10.1523/JNEUROSCI.2440-20.2021

Figure 1.

Figure 1.

Functional validation of Oprm1 haploinsufficiency. A, Breeding strategy used to generate littermates of all possible genotypes for validation experiments (top), with the legend defining the appearance of individual data points for each genotype and sex (bottom). B, Assessment of μ-opioid receptor (Oprm1) mRNA levels in nucleus accumbens tissue punches using quantitative PCR in Oprm1+/+ (n = 14), Oprm1+/− (n = 15), and Oprm1−/− (n = 9) mice. C, D, Distance traveled in a test of open field activity (C) and thermal antinociception on the hot plate (D) after the injection of morphine in Oprm1+/+ (n = 12), Oprm1+/− (n = 10), and Oprm1−/− (n = 11) mice. All groups contained similar numbers of female mice (open symbols) and male mice (closed symbols). *p < 0.05 between groups, LSD post hoc test.