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Orientia tsutsugamushi transcriptionally expresses ompA, tsa22 and tsa56 during infection of mammalian host cells. HeLa, RF/6A or THP-1 cells were synchronously infected with O. tsutsugamushi (Ot) followed by collection of total RNA at 24, 48 or 72 h. RT-qPCR was performed using gene-specific primers. Relative ompA-, tsa22- and tsa56-to-Ot 16S rRNA gene (ott16S) expression was determined using the 2−ΔΔCT method. Data are mean values ± SD from three experiments performed in triplicate.
