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. 2021 Sep 13;79(7):ftab044. doi: 10.1093/femspd/ftab044

Figure 2.

Figure 2.

The UTR of ompA, tsa22 and tsa56 each contains a predicted RBS and −10 and −35 sequences. Upstream sequences of ompA(A), tsa22(B) and tsa56(C) were evaluated for the presence of RBS and −10 and −35 sequences. Initiation codons are indicated by white boldface text and purple highlighting. Putative RBS sequences are underlined. (A) The ompA UTR −10 and −35 sequences are denoted by boldface text. The two sets of −10 and −35 sequences in the UTRs of tsa22 (B) and tsa56 (C), which are designated ‘down’ and ‘up’ based on proximity to the start codon, are indicated by blue and red boldface text, respectively. Nucleotides cloned into pPROBE-NT for assessing promoter activity of tsa22-down and tsa56-down are highlighted gray, while those for evaluating promoter activity of tsa22-up and tsa56-up are highlighted yellow.