Biological sample (Homo sapiens) |
PBMC samples from HIV-infected individuals |
|
|
|
Commercial assay or kit |
DNA-free DNA Removal Kit |
ThermoFisher Scientific |
Cat# AM1906 |
|
Commercial assay or kit |
Platinum Quantitative PCR SuperMix-UDG |
ThermoFisher Scientific |
Cat# 11730–025 |
|
Commercial assay or kit |
TaqMan β-Actin Detection Reagents |
ThermoFisher Scientific |
Cat# 401846 |
|
Commercial assay or kit |
TaqMan Ribosomal RNA Control Reagents |
ThermoFisher Scientific |
Cat# 4308329 |
|
Chemical compound, drug |
SuperScript III reverse transcriptase |
ThermoFisher Scientific |
Cat# 18080–085 |
|
Chemical compound, drug |
Random primers |
ThermoFisher Scientific |
Cat# 48190–011 |
|
Chemical compound, drug |
RNaseOUT Recombinant Ribonuclease Inhibitor |
ThermoFisher Scientific |
Cat# 10777–019 |
|
Software, algorithm |
Prism 8.3.0 |
GraphPad Software |
https://www.graphpad.com/; RRID:SCR_002798
|
|
Software, algorithm |
IBM SPSS Statistics (version 25) |
IBM Corporation |
https://www.ibm.com/; RRID:SCR_019096
|
|