| gene (Danio rerio) |
plin2 |
Ensembl GRCz11 |
ENSDARG00000042332; plin2-203 ENSDART00000175378.2 |
|
| gene (Danio rerio) |
zgc:77,486(plin3) |
Ensembl GRCz11 |
ENSDARG00000013711; zgc:77,486 ENSDART00000100473.5 |
|
| Gene (Homo sapiens) |
PLIN2 |
Ensembl GRCh38.p13 |
ENSG00000147872; PLIN2-201 ENST00000276914.7 |
|
| Gene (Homo sapiens) |
PLIN3 |
Ensembl GRCh38.p13 |
ENSG00000105355; PLIN3-201 PLIN3-201 ENST00000221957.9 |
|
| Strain, strain background (Danio rerio) |
AB
|
Zfin |
ZDB-GENO-960809–7 |
|
| Strain, strain background (Danio rerio) |
Fus(EGFP-plin2)
|
This paper |
|
Available on request from Steven Farber |
| Strain, strain background (Danio rerio) |
Fus(plin3-RFP)
|
This paper |
|
Available on request from Steven Farber |
| Strain, strain background (Danio rerio) |
Tg(fabp2:EGFP-PLIN2)
|
This paper |
|
Zebrafish fabp2 promoter, human PLIN2 coding sequence; Available on request from Steven Farber |
| Strain, strain background (Danio rerio) |
Tg(fabp10a: EGFP-PLIN2)
|
This paper |
|
Zebrafish fabp10a promoter, human PLIN2 coding sequence; Available on request from Steven Farber |
| Strain, strain background (Danio rerio) |
Tg(hsp70l: EGFP-PLIN2)
|
This paper |
|
Zebrafish hsp70l promoter, human PLIN2 coding sequence; Available on request from Steven Farber |
| Strain, strain background (Danio rerio) |
Tg(fabp2:PLIN3-EGFP)
|
This paper |
|
Zebrafish fabp2 promoter, human PLIN3 coding sequence; Available on request from Steven Farber |
| Strain, strain background (Danio rerio) |
Tg(hsp70: PLIN3-EGFP)
|
This paper |
|
Zebrafish hsp70l promoter, human PLIN3 coding sequence; Available on request from Steven Farber |
| Recombinant DNA reagent |
pCRII(plin3_900)(plasmid) |
This paper |
|
Zebrafish plin3 (zgc:77486) cDNA clone for in situ probe; Available on request from Steven Farber |
| Recombinant DNA reagent |
pCRII(plin2)(plasmid) |
This paper |
|
Zebrafish plin2 cDNA clone for in situ probe; Available on request from Steven Farber |
| Recombinant DNA reagent |
Pk-GoldyTal(plin2 Pair 1 LeftTAL 1)(plasmid) |
This paper |
|
TALEN targets: TTTCTGCTAACATGG; Available on request from Steven Farber |
| Recombinant DNA reagent |
Pk-GoldyTal(plin2 Pair 1 RightTAL 2)(plasmid) |
This paper |
|
TALEN target: AAATAACCAGGTTTGCC; Available on request from Steven Farber |
| Recombinant DNA reagent |
Pk-GoldyTal(plin2 Pair 2 LeftTAL 1)(plasmid) |
This paper |
|
TALEN target: TTTCTGCTAACATGGGT; Available on request from Steven Farber |
| Recombinant DNA reagent |
Pk-GoldyTal(plin2 Pair 2 RightTAL 2)(plasmid) |
This paper |
|
TALEN target: AATAACCAGGTTTGCC; Available on request from Steven Farber |
| Recombinant DNA reagent |
Pk-GoldyTal(plin3 Pair 1 LeftTAL 1) (plasmid) |
This paper |
|
TALEN target: TTGCGCCTCAGATAAC; Available on request from Steven Farber |
| Recombinant DNA reagent |
Pk-GoldyTal(plin3 Pair 1 RightTAL 2) (plasmid) |
This paper |
|
TALEN target: AATTGCCACACAACCT; Available on request from Steven Farber |
| Recombinant DNA reagent |
Pk-GoldyTal(plin3 Pair 2 LeftTAL 1) (plasmid) |
This paper |
|
TALEN target:CAGATAACAGAGAAA; Available on request from Steven Farber |
| Recombinant DNA reagent |
Pk-GoldyTal(plin3 Pair 2 RightTAL 2) (plasmid) |
This paper |
|
TALEN target:CACACAACCTAAATA; Available on request from Steven Farber |
| Recombinant DNA reagent |
pKT3Ts-goldyTALEN vector |
Welker et al., 2016
|
Addgene plasmid #80330; RRID:Addgene_80330
|
|
| Recombinant DNA reagent |
FusX TALEN assembly system |
Ma et al., 2013; Ma et al., 2016
|
Addgene kit #1000000063 |
|
| Recombinant DNA reagent |
pDestTol2Pa2(plasmid) |
Kwan et al., 2007
|
Plasmid #394 |
http://tol2kit.genetics.utah.edu
|
| Recombinant DNA reagent |
P4-P1R_zPLIN2_5’UTR(plasmid) |
This paper |
|
5’ entry plasmid for left homology arm of pDestTol2Pa2(EGFP-plin2) donor plasmid V2; Available on request from Steven Farber |
| Recombinant DNA reagent |
pME-EGFP no stop 12aa linker(plasmid) |
This paper |
|
EGFP middle entry plasmid for pDestTol2Pa2(EGFP-plin2) donor plasmid V2Includes strong zebrafish kozak sequence; Available on request from Steven Farber |
| Recombinant DNA reagent |
P3R-P3-zPLIN2_1–900 |
This paper |
|
3’ entry plasmid for right homology arm of pDestTol2Pa2(EGFP-plin2) donor plasmid V2; Available on request from Steven Farber |
| Recombinant DNA reagent |
pDestTol2Pa2(EGFP-plin2) donor plasmid V2 |
This paper |
|
Donor plasmid for creation of Fus(EGFP-plin2) fish |
| Recombinant DNA reagent |
P4-P1R_zPLIN3_last679 |
This paper |
|
5’ entry plasmid for left homology arm of pDestTol2Pa2(PLIN3-RFP); Available on request from Steven Farber |
| Recombinant DNA reagent |
tagRFP-t coding sequence |
Horstick et al., 2015
|
|
Modified for zebrafish from Addgene #61390
|
| Recombinant DNA reagent |
pME-tagRFP-t (plasmid) |
This paper |
|
Middle entry plasmid for pDestTol2Pa2(PLIN3-RFP); Available on request from Steven Farber |
| Recombinant DNA reagent |
P2R_P3_zPLIN3_3’UTR |
This paper |
|
3’ entry plasmid for right homology arm of pDestTol2Pa2(PLIN3-RFP); Available on request from Steven Farber |
| Recombinant DNA reagent |
pDestTol2Pa2(plin3-RFP) donor plasmid |
This paper |
|
Donor plasmid for creation of Fus(plin3-RFP) fish; Available on request from Steven Farber |
| Recombinant DNA reagent |
pDestTol2Pa2(fabp2: EGFP-PLIN2)(plasmid) |
This paper |
|
Zebrafish fabp2 promoter, human PLIN2 coding sequence; Available on request from Steven Farber |
| Recombinant DNA reagent |
pDestTol2Pa2(fabp10a: EGFP-PLIN2)(plasmid) |
This paper |
|
Zebrafish fabp10a promoter, human PLIN2 coding sequence; Available on request from Steven Farber |
| Recombinant DNA reagent |
pDestTol2Pa2(hsp70l: EGFP-PLIN2)(plasmid) |
This paper |
|
Zebrafish hsp70l promoter, human PLIN2 coding sequence; Available on request from Steven Farber |
| Recombinant DNA reagent |
pDestTol2Pa2(fabp2: PLIN3-EGFP)(plasmid) |
This paper |
|
Zebrafish fabp2 promoter, human PLIN3 coding sequence; Available on request from Steven Farber |
| Recombinant DNA reagent |
pDestTol2Pa2(hsp70l: PLIN3-EGFP)(plasmid) |
This paper |
|
Zebrafish hsp70l promoter, human PLIN3 coding sequence; Available on request from Steven Farber |
| Recombinant DNA reagent |
pGFP-hADRP(plasmid)(plasmid) |
Targett-Adams et al., 2003
|
|
Human PLIN2 coding sequence (Alternative name ADRP) in pEGFP-C1 vector |
| Recombinant DNA reagent |
pCR8(pGFP-hADRP)(plasmid) |
This paper |
|
Human PLIN2 coding sequence (Alternative name ADRP); Available on request from Steven Farber |
| Recombinant DNA reagent |
pDNR-Dual(h Perilipin 3) (plasmid)(TIP47) |
FLEXgene Repository (Harvard Medical School) |
Clone ID:HsCD00004695 |
Human PLIN3 coding sequence Alternative name: TIP47 |
| Recombinant DNA reagent |
pCR8(Perilipin3)(TIP47)(plasmid) |
This paper |
|
Middle entry plasmid, Human PLIN3 coding sequence Alternative name: TIP47; Available on request from Steven Farber |
| Recombinant DNA reagent |
Zebrafish fabp2 promoterp5E fabp2 (–2.3 kb) fabp2 (plasmid) |
Park et al., 2019
|
|
5’ entry plasmid, zebrafish fabp2 (ifabp) promoter sequence |
| Recombinant DNA reagent |
p5E fabp10a, (–2.8 kb) |
Her et al., 2003
|
|
zebrafish fabp10a (lfabp) promoter sequence |
| Recombinant DNA reagent |
p5E-hsp70lheat shock cognate 70 kDa protein, like |
Kwan et al., 2007
|
Plasmid #222 |
http://tol2kit.genetics.utah.edu
|
| Recombinant DNA reagent |
p3E-EGFPpA |
Kwan et al., 2007
|
Plasmid #366 |
http://tol2kit.genetics.utah.edu
|
| Recombinant DNA reagent |
pME-EGFP no stop |
Kwan et al., 2007
|
Plasmid #455 |
http://tol2kit.genetics.utah.edu
|
| Recombinant DNA reagent |
p3E-polyA |
Kwan et al., 2007
|
Plasmid #302 |
http://tol2kit.genetics.utah.edu
|
| Sequence-based reagent |
PCR primers |
This paper |
|
Please refer to Supplementary file 1
|
| Commercial assay or kit |
T3 Message Machine kit |
Thermo Fisher Scientific |
Catalog #AM1348 |
|
| Commercial assay or kit |
DIG RNA labelling kit |
Roche |
Catalog#11277073910 |
|
| Other |
T7 polymerase |
Roche |
Catalog #10881767001 |
|
| Other |
SP6 polymerase |
Roche |
Catalog #10810274001 |
|
| Commercial assay or kit |
TA Cloning Kit, Dual Promoter, with pCRII-TOPO vector |
Thermo Fisher Scientific |
Catalog # K207020 |
|
| Commercial assay or kit |
REDExtract-N-Amp Tissue PCR kit |
Sigma-Aldrich |
Catalog #XNAT |
|
| Commercial assay or kit |
RNA Clean and Concentrator Kit |
Zymo Research |
Catalog #R1013 |
|
| Commercial assay or kit |
iScript cDNA Synthesis Kit |
Bio-Rad Laboratories, Inc. |
Catalog #1708891 |
|
| Commercial assay or kit |
SsoAdvanced Universal SYBR Green Supermix |
Bio-Rad Laboratories, Inc. |
Catalog #1725271 |
|
| Commercial assay or kit |
MultiSite gateway assembly system |
Invitrogen |
Catalog #12537–023 |
|
| Commercial assay or kit |
pCR8/GW/TOPO TA cloning kit |
Thermo FisherScientific |
Catalog # K250020 |
|
| Other |
BODIPY (558/568)-C12 |
Thermo Fisher Scientific |
Catalog #D3835 |
4 µg/ml |
| Other |
BODIPY FL-C12 |
Thermo Fisher Scientific |
Catalog #D3822 |
4 µg/ml |
| Other |
Cholesterol |
Sigma-Aldrich |
Catalog#C8667 |
4% w/w |
| Other |
HSC LipidTOX Green |
Thermo Fisher Scientific |
Catalog #H34475
|
1:5,000 |
| Other |
HSC LipidTOX Red |
Thermo Fisher Scientific |
Catalog #H34476
|
1:5,000 |
| Other |
RNAlater
|
Thermo Fisher Scientific |
Catalog # AM7020 |
|
| Other |
FokI restriction enzyme |
New England Biolabs |
Catalog # R0109 |
|
| Other |
HphI restriction enzyme |
New England Biolabs |
Catalog # R0158 |
|
| Other |
Gemma Micro 75, 150 & 500 |
Skretting |
|
Zebrafish diet |
| Software, algorithm |
Mojo Hand design tool |
Neff et al., 2013
|
|
|
| Software, algorithm |
Fiji |
Schindelin et al., 2012
|
(Fiji, RRID:SCR_002285) |
|
| Software, algorithm |
ilastik |
Berg et al., 2019
|
Ilastik, RRID:SCR_015246
|
|
| Software, algorithm |
GraphPad Prism 9 |
GraphPad |
GraphPad Prism, RRID:SCR_002798
|
|
