Figure 7.

Most RNAi clones that decrease mitochondrial fragmentation do not cause a decrease in polyglutamine aggregation. BW-40Q worms were treated with RNAi against genes which decrease mitochondrial fragmentation in C. elegans when knocked down via RNAi. Animals were imaged as day 1 adults. Of the 26 RNAi clones tested, four RNAi clones resulted in a small but significant decrease in total aggregates per worm: drp-1, abhd-11.1, E04A4.4, and C33A12.1. Grey bars show BW-40Q worms treated with empty vector (EV). Green bars show RNAi clones that significantly decreased aggregate number. None of the RNAi clones resulted in increased aggregation. Bars indicate the mean value. Significance was assessed using one-way ANOVA. Error bars indicate SEM. *p<0.05, **p<0.01, ***p<0.001.