Skip to main content
. 2021 Sep 1;41(5):749–762. doi: 10.5851/kosfa.2021.e33

Fig. 2. Effect of fermented colostrum whey on AQP-3 expression.

Fig. 2.

HaCaT cells were treated with different concentrations (1, 10, and 100 μg/mL) of (A) colostrum whey, (B) milk whey, (C) fermented colostrum whey, and (D) fermented milk whey, for 3 h. AQP-3 mRNA expression was analyzed by RT-PCR. GAPDH was used as a control. (E) AQP-3 mRNA levels were quantified by real-time PCR (QPCR). GAPDH mRNA level was used for the normalization of the mRNA levels of AQP-3. (F) HaCaT cells were treated with a concentration of 100 μg/mL of fermented colostrum whey, and the AQP-3 mRNA levels at different durations (1, 2, 3, 6, and 9 h) were detected by QPCR. GAPDH mRNA levels were used for normalization. Data were presented as the means±SE values of three independent experiments (n=3).