Figure 2.

S-D614G is highly stable over a broad range of temperatures. Representative NSEM micrographs of S-D614 (top panels) and S-D614G (bottom panels) after different temperature treatments. Selected 2D classes of picked particle images are shown below each panel. All micrographs and 2D classes are shown in the same scales. The scale bars of the micrograph and the 2D classes are indicated on the lower left corners of the Day 0 datasets. Significant unfolding of S-D614 was observed after heat shocks and after 6 days of incubation at 4 °C. In contrast, S-D614G exhibited visible unfolding only by heat shock at 60 °C for 30 min 3D maps of the individual samples derived from the native-like particle images are shown on the upper right corner of each panel.