Figure 4.

Relative native-like particle number of S-D614G as a function of temperature. All samples were incubated at the specified temperatures as indicated along the X-axis for 30 min prior to NESM grid preparation. The numbers of native-like particles in individual micrographs were normalized with respect to the average value of freshly prepared sample (37 °C, day 0). The error bars correspond to the standard deviations of the numbers within the micrographs collected under the same conditions. For each temperature, between 50 and 60 NSEM micrographs were collected. 3D maps of the individual samples derived from the native-like particle images are shown next to the data points.