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. 2021 Sep 13;17(10):1075–1083. doi: 10.1038/s41589-021-00858-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a, Close-up of the noncovalent interface between NEDD8 (yellow) and its linked CUL5 WHB domain (green), from the cryo-EM structure of neddylated CRL5^Vif-CBFβ-A3C complex with ARIH2*. Side-chains mediating contacts, and CUL5 H29-, H30-, and H31-helices are indicated. b, Portion of the cryo-EM structure corresponding to NEDD8, CUL5 C-terminal domain (encompassing the 4HB, C/R domain, and WHB domain), and the N-terminal strand of RBX2 contributing to the C/R domain. c, Lattice packing in prior crystal structure of neddylated CUL5 C-terminal domain (CTD)-RBX1 complex¹², highlighting CUL5’s H29-helix and WHB domain in dark green, and its linked NEDD8 in yellow. Dotted line indicates the border between the two different neddylated CUL5 CTD-RBX1 complexes in the crystal lattice. NEDD8 and CUL5 interact similarly as in the cryo-EM structure, although contacts between NEDD8 and its linked CUL5 WHB domain are with the C/R domain of the adjacent complex in the crystal.