. 2021 Aug 3;22(5):e68. doi: 10.4142/jvs.2021.22.e68

Table 3. Primers used in this study.

Target		Primer	Primer sequenc (5′-3′)	Product size (bp)	Reference
MCR
	Multiplex	mcr1-F	AGTCCGTTTGTTCTTGTGGC	320	[24]
		mcr1-R	AGATCCTTGGTCTCGGCTTG	320	[24]
		mcr2-F	CAAGTGTGTTGGTCGCAGTT	715	[24]
		mcr2-R	TCTAGCCCGACAAGCATACC	715	[24]
		mcr3-F	AAATAAAAATTGTTCCGCTTATG	929	[24]
		mcr3-R	AATGGAGATCCCCGTTTTT	929	[24]
		mcr4-F	TCACTTTCATCACTGCGTTG	1116	[24]
		mcr4-R	TTGGTCCATGACTACCAATG	1116	[24]
		mcr5-F	ATGCGGTTGTCTGCATTTATC	1644	[24]
		mcr5-R	TCATTGTGGTTGTCCTTTTCTG	1644	[24]
Carbapenemase genes
	Multiplex	bla_IMP-F	GGAATAGAGTGGCTTAAYTCTC	232	[25]
		bla_IMP-R	GGTTTAAYAAAACAACCACC	232	[25]
		bla_VIM-F	GATGGTGTTTGGTCGCATA	390	[25]
		bla_VIM-R	CGAATGCGCAGCACCAG	390	[25]
		bla_OXA-F	GCGTGGTTAAGGATGAACAC	477	[25]
		bla_OXA-R	CATCAAGTTCAACCCAACCG	477	[25]
		bla_NDM-F	GGTTTGGCGATCTGGTTTTC	621	[25]
		bla_NDM-R	CGGAATGGCTCATCACGATC	621	[25]
		bla_KPC-F	CGTCTAGTTCTGCTGTCTTG	798	[25]
		bla_KPC-R	CTTGTCATCCTTGTTAGGCG	798	[25]
PBRT
	Multiplex 1	HI1-F	GGAGCGATGGATTACTTCAGTAC	471	[27]
		HI1-R	TGCCGTTTCACCTCGTGAGTA	471	[27]
		HI2-F	TTTCTCCTGAGTCACCTGTTAACAC	644	[27]
		HI2-R	GGCTCACTACCGTTGTCATCCT	644	[27]
		I1-F	CGAAAGCCGGACGGCAGAA	139	[27]
		I1-V	TCGTCGTTCCGCCAAGTTCGT	139	[27]
	Multiplex 2	X-F	AACCTTAGAGGCTATTTAAGTTGCTGAT	376	[27]
		X-R	TGAGAGTCAATTTTTATCTCATGTTTTAGC	376	[27]
		L/M-F	GGATGAAAACTATCAGCATCTGAAG	785	[27]
		L/M-R	CTGCAGGGGCGATTCTTTAGG	785	[27]
		N-F	GTCTAACGAGCTTACCGAAG	559	[27]
		N-R	GTTTCAACTCTGCCAAGTTC	559	[27]
	Multiplex 3	FIA-F	CCATGCTGGTTCTAGAGAAGGTG	462	[27]
		FIA-R	GTATATCCTTACTGGCTTCCGCAG	462	[27]
		FIB-F	GGAGTTCTGACACACGATTTTCTG	702	[27]
		FIB-R	CTCCCGTCGCTTCAGGGCATT	702	[27]
		W-F	CCTAAGAACAACAAAGCCCCCG	242	[27]
		W-R	GGTGCGCGGCATAGAACCGT	242	[27]
	Multiplex 4	Y-F	AATTCAAACAACACTGTGCAGCCTG	765	[27]
		Y-R	GCGAGAATGGACGATTACAAAACTTT	765	[27]
		P-F	CTATGGCCCTGCAAACGCGCCAGAAA	534	[27]
		P-R	TCACGCGCCAGGGCGCAGCC	534	[27]
		FIC-F	GTGAACTGGCAGATGAGGAAGG	262	[27]
		FIC-R	TTCTCCTCGTCGCCAAACTAGAT	262	[27]
	Multiplex 5	A/C-F	GAGAACCAAAGACAAAGACCTGGA	465	[27]
		A/C-R	ACGACAAACCTGAATTGCCTCCTT	465	[27]
		T-F	TTGGCCTGTTTGTGCCTAAACCAT	750	[27]
		T-R	CGTTGATTACACTTAGCTTTGGAC	750	[27]
		FIIS-F	CTGTCGTAAGCTGATGGC	270	[27]
		FIIS-R	CTCTGCCACAAACTTCAGC	270	[27]
	Simplex 1	FrepB-F	TGATCGTTTAAGGAATTTTG	270	[27]
	Simplex 1	FrepB-R	GAAGATCAGTCACACCATCC	270	[27]
	Simplex 2	K-F	GCGGTCCGGAAAGCCAGAAAAC	160	[27]
	Simplex 2	K-R	TCTTTCACGAGCCCGCCAAA	160	[27]
	Simplex 3	B/O-F	GCGGTCCGGAAAGCCAGAAAAC	159	[27]
	Simplex 3	B/O-R	TCTGCGTTCCGCCAAGTTCGA	159	[27]
	MLST	fimA-F	TCAGGGGAGAAACAGAAAACTAAT	760	[28]
		fimA-R	TCCCCGATAGCCTCTTCC	760	[28]
		manB-F	CCGGCACCGAAGAGA	893	[28]
		manB-R	CGCCGCCATCCGGTC	893	[28]
		mdh-F	ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG	849	[28]
		mdh-R	ATATCTTTYTTCAGCGTATCCAGCAT	849	[28]

PBRT, polymerase chain reaction-based replicon typing; MLST, multi-locus sequence typing.