FIGURE 8.

Rifampicin mutagenesis in R391 and R391ΔcroS strains. MVG114, MVG114/R391, and MVG114/R391ΔcroS cultures were started from with approximately 1,000 cells, or less, and grown overnight to stationary phase. Cells were plated onto LB agar plates containing rifampicin to select for rifampicin‐resistant mutants. Appropriate dilutions were plated to LB agar plates to determine viable counts, and the frequency of mutagenesis to rifampicin resistance was calculated. Error bars represent the standard error of the mean (SEM). The MVG114/R391ΔcroS strain exhibits an ~10‐fold higher frequency of mutagenesis to rifampicin resistance than the MVG114/R391 strain