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. 2021 Jul 12;116(3):877–889. doi: 10.1111/mmi.14777

TABLE 3.

Oligonucleotides used in this study

Name Sequence Source
P1 a CTCGCTTTTGTGTTTACGTAAAATGCTGACCATTATTTGCCCTTAATCCTAAATTAACCCTCACTAAAGGGCGG BioSpring
P2 a CAGTTTAAACTGTAACATTCAATCTTGTAACAGTTATTATTGTAACAGGAGACGGTTAGCACGGAGTTCATTAGGGCTC Biospring
cp01 b GCGATGCCATACCAATGAAGTCG BioSpring
cp02 b CAACGTAACCAATCGGCAGTCG BioSpring
cp03 b CCAGTGACTTCACGCCACTCC BioSpring
cp04 b CAGGATCAACAACGATCACTGCC BioSpring
a

Used in the generation of linear knock out cassette.

b

Used in the amplification of the modified region and subsequent DNA sequencing.