Fig 3. Conversion of QoxABCD to a bo₃ quinol oxidase suppresses Mn sensitivity.

A) QoxABCD is the major cytochrome in WT and PS cells, and mutation of qoxA leads to a loss of characteristic reddish-brown color as observed in cell pellets. B) Pathway of heme B conversion to heme A by the sequential action of CtaB and CtaA. Heme C is generated as thioester linkage of heme B in polypeptides. Additionally, heme D is also produced from heme B. C) Mn sensitivity enumerated on MM-malate plates. Dotted lines highlight strains with similar levels of Mn sensitivity. All measurements are mean±SEM (n=3). **** is P<0.0001 and ns, no statistical significance as determined using one-way ANOVA test with Tukey’s post-hoc analysis. All comparisons are shown with respect to PS.