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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Mol Microbiol. 2021 Jul 4;116(3):827–840. doi: 10.1111/mmi.14773

Figure 2. In vitro interaction of DRBD18 with Mex67 and Mtr2.

Figure 2.

(A) Recombinant His-tagged Mtr2, GST-tagged DRBD18, and GST were expressed, purified by affinity chromatography, and analyzed by 12.5% SDS-PAGE followed by Coomassie brilliant blue staining. Migration of molecular weight standards (kDa) is indicated on the left. (B) Recombinant GST-DRBD18 or GST were immobilized on glutathione-Sepharose 4B beads, followed by incubation with His-Mtr2 for GST pulldown assays. Western blot analyses were performed using anti-His and anti-GST antibodies to detect His-Mtr2 and GST or GST-DRBD18, respectively. Purified His-Mtr2 (in lane 1), and GST-DRBD18 or GST on glutathione-Sepharose 4B beads incubated without His-Mtr2 were used as inputs for the experiments (in lane 2 and 3 respectively). Two biological replicates, each with two technical replicates, were performed, and a representative experiment is shown.