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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Prostate. 2021 Aug 16;81(15):1159–1171. doi: 10.1002/pros.24210

Figure 5. Proteomic profiling of androgen dependent and castration resistant prostate cancer cell lines.

Figure 5.

A. Western blot analysis showing AR and NKX3.1 expression as well as abundance of phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) and Phospho-Akt (Ser473) in LNCaP, LNCaP-abl, LAPC4, LAPC4-CR, VCaP, VCaP-CR. B. Principal component analysis of SWATH-MS data from LNCaP, LNCaP-abl, LAPC4, LAPC4-CR, VCaP, VCaP-CR reveals clustering of cell line pairs according to their parental origin. C. Heatmap of paired comparisons of differentially expressed proteins in parental and castration resistant cell line, red indicates higher expression in castration resistant model, blue indicates higher expression in the parental line. D. Correlation between mRNA (x-axis) and protein (y-axis) expression in parental and castration resistant cell line pairs. Names of significantly differentially expressed proteins are indicated. Proteins printed in red are further validated by western blot analysis. E. Differential expression of ATG3, GSTP1 and ANXA2 in LAPC4, LAPC4-CR, VCaP and VCaP-CR cells. Bands show immunoreactivity in western blot. Boxes (top row) indicate mRNA expression based on RNA-seq (red = high expression, white low/no expression); boxes (second row) indicate protein expression based on SWATH-MS (blue = high expression, white low/no expression). Beta-actin serves as loading control.