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. 2021 Sep 23;11:18910. doi: 10.1038/s41598-021-98082-w

Figure 2.

Figure 2

Pyruvate starvation induces rapid cell death of primary cultured rat DRG neurons, NSC-34 cells, MES13 cells and HAEC cells under high-glucose conditions. (A-D) Representative phase-contrast micrographs of DRG neurons at 24 h [Glc 5 mM/Pyr (+)] (A), [Glc 5 mM/Pyr (−)] (B), [Glc 50 mM/Pyr ( +)] (C), and [Glc 50 mM/Pyr (−)] (D) groups. Scale bar represents 100 μm. (E) The viability of DRG neurons at 3 and 6 h in the [Glc 5 mM/Pyr (+)] (blue), [Glc 5 mM/Pyr (−)] (yellow), [Glc 50 mM/Pyr (+)] (brown), and [Glc 50 mM/Pyr (−)] (green) groups was determined by Trypan blue staining. (F–H) The viability of NSC-34 cells (F), MES13 cells (G) and HAEC cells (H) at 24 h after exposure to the 4 conditions described above was determined by MTS assay. Values represent mean + SD from three (E) and six (F–H) experiments (individual values are depicted as circles, triangles, pluses and crosses). * P < 0.05, ** P < 0.01.