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. 2021 Sep 23;11:18910. doi: 10.1038/s41598-021-98082-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pyruvate starvation under high-glucose conditions induces ROS production, but not lipid peroxidation. The quantity of MDA (A) and the fluorescent intensity of CellROX (B) in IMS32 cells at 1 h in the [Glc 5 mM/Pyr (+)] (blue), [Glc 5 mM/Pyr (-)] (yellow), [Glc 15 mM/Pyr (+)] (brown) and [Glc 15 mM/Pyr (-)] (green) was determined by TBARS assay (A) and CellROX assay (B), respectively. The fluorescent intensity of CellROX was normalized to ratio of the value of [Glc 5 mM/Pyr (+)]. Values represent mean + SD from three (A) and six (B) experiments (individual values are depicted as circles, triangles, pluses and crosses). ** P < 0.01.