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. 2021 Sep 23;4:1124. doi: 10.1038/s42003-021-02624-x

Fig. 1. Experimental set-up to understand the formation of the Lewy bodies by measuring protein–protein interactions along the aggregation cascade of α-SYN.

Fig. 1

a The Lewy bodies contain >80 different proteins, which are involved in several cell processes. Inset adapted from Brown et al.9. b By using a Gateway cloning system, a library of N-terminal GFP-tagged Lewy body proteins was acquired for expression in the cell-free Leishmania tarentolae lysate. c On pathway to its inclusion in the Lewy bodies, the aggregation of α-SYN is known for comprising different steps. Upon misfolding of its monomeric form, α-SYN self-assembles into oligomeric, pre-fibrillar and fibrillar forms, respectively. Three different assays were designed to identify the main interactors of α-SYN in four of its forms along this cascade: monomeric (AlphaScreen), oligomeric, pre-fibrillar, and fibrillar (two-colour coincidence detection using a single-molecule spectroscopy set-up).