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. 2021 Sep 23;4:1124. doi: 10.1038/s42003-021-02624-x

Fig. 3. Interactors of oligomeric forms of alpha-synuclein.

Fig. 3

a The pathological mutants of α-SYN (A30P, G51D and A53T) were tagged with mCherry in their C-termini and co-expressed in LTE with the library of LB proteins. Shaded circles represent the confocal volume (1 fL). Four 30 s traces were acquired for each co-expression and TCCD shows binding to synuclein aggregates. For all traces, GFP signal is represented in positive axis, mCherry signal in negative axis. (i) A negative control of superfolded GFP was used. (ii) The same mutant form of synuclein, C-GFP tagged, served as a positive control. (iii) Association Quotient (Q) was calculated for each trace as the number of coincident events (C) over the total number of events, taking chance coincidence (E) into account. Averages of four measurements were acquired and plotted as bar plots and heatmaps for: A30P (b), G51D (c) and A53T (d). Dotted line represents mean + SEM of GFP control. Dunnett’s multiple comparisons test against co-expression with GFP. #p ≤ 0.0001, p ≤ 0.001, p ≤ 0.01, ¥p ≤ 0.05, n = 4. Error bars show mean ± SEM. Proteins are displayed in alphabetical order.