FIG. 1.

Stimulation of p65-dependent transactivation requires multiple functional domains of CBP. (A) Schematic of CBP wild-type (wt) and mutant expression constructs used in the transient transfection assays. (B) Expression of CBP deletion mutants block p65 transactivation. COS-7 cells were transiently transfected with 1 μg of −578 E-selectin-CAT, 250 ng of pcDNA-p65 (lanes 2 to 10), and either 1 or 10 μg of the indicated CBP expression construct. Forty-eight hours posttransfection, the cells were harvested and CAT activity was assayed as described in Materials and Methods. The data are representative of three independent experiments performed in duplicate. (C) Schematic of E1A wild-type and mutant expression constructs used in the transient transfection assays. (D) Differential effects of mutated forms of E1A on p65-stimulated reporter gene expression. COS-7 cells were transiently transfected with 1 μg of −578 E-selectin-CAT, 250 ng of pcDNA-p65, and the indicated concentrations of the wild-type E1A, E1Ad2-36, or E1AH3N expression construct. Forty-eight hours posttransfection, the cells were harvested and CAT activity was assayed as described in Materials and Methods. The data are representative of three independent experiments performed in duplicate.