FIG. 4.

NF-κB-dependent transactivation in vitro requires specific functional domains of SRC-1/NCoA-1. (A) Schematic representation of SRC-1/NCoA-1 expression plasmids used in the overexpression experiments. (B) The N terminus of SRC-1/NCoA-1 is required for potentiation of p65-dependent transactivation. COS-7 cells were transiently transfected with 1 μg of −578 E-selectin-CAT, 100 ng of pcDNA-p65, and 0.5, 1, 4, or 8 μg of either wild-type CMV-SRC-1/NCoA-1 (WT), CMV-SRC-1-NR/CBP, or CMV-SRC-1 ΔN. Forty-eight hours posttransfection, the cells were harvested and CAT activity was assayed as described in Materials and Methods. The level of activity observed upon cotransfection of E-selectin CAT and p65 was set at one. Data are presented as means; error bars, standard deviations.