Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 23;11:18916. doi: 10.1038/s41598-021-97928-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Differentiation of iPSCs to i-motor neurons. (a) Representative phase contrast image showing derivation of i-motor neurons from iPSCs to involve the embryoid body stage using small molecules and growth factors. (b) qPCR demonstrated expression of motor neuron markers HB9, ChAt and SMI32 in all lines of iPSCs-derived motor neurons. Gene expression was normalized to GAPDH and expressed relative to iNPCs and log10 of fold change values are presented in Heat map generated using Graphpad Prism version 7.03. n = 3 per line, with 3 unique human lines per group., data represent mean of the 3 unique lines +/− SEM. (c) Immunofluorescence staining in i-motor neurons shows positive expression for motor neuron markers HB9, βIII-tubulin, ChAT, and SMI32 and were quantified using Image J software.