FIG. 2.

Cyclin E is a direct target of E2F-1. ERE2F-1 clone D cells were made quiescent by growing the cells in medium with 0.1% serum for 48 h. RT-PCR was performed on samples of total RNA from ERE2F-1 clone D that had been kept for 48 h in medium containing 0.1% serum and induced for different lengths of time by addition of OHT, serum, or OHT plus CHX. Primers specific for the amplification of cyclin E mRNA were used as described in Materials and Methods.