Fig. 6.
Comparison of the influence of a 1 h LPS (+LBP for in vitro experiments) preincubation on the IL-8 induced changes in FSC, pHi, and glucose metabolism between an ex vivo whole blood model and an in vitro model. The delta ± SD between IL-8-stimulated cells and the respective control are shown. a LPS caused a significant reduction of the IL-8-induced change in the FSC both in vitro and ex vivo, mainly by raising the control level; n = 6; *p < 0.05 (Mann-Whitney test). b LPS creates an intracellular alkalization and disrupts the pH shift elicited by IL-8; n = 6 *p < 0.05 (Mann-Whitney test). c Interaction of LPS with the glucose uptake and reduction of the IL-8-induced increase; n = 4–6 *p < 0.05 (Mann-Whitney test). d Downregulation of IL-8 receptors CXCR1 (CD181) and CXCR2 (CD182) on the cell surface by LPS treatment for 1 h; n = 6; *p < 0.05, **p< 0.01 versus Ctrl (one-way ANOVA followed by Dunn's multiple comparisons test). IL, interleukin; pHi, intracellular pH; LBP, LPS-binding protein; FSC, forward scatter area; Ctrl, control.