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Induction of apoptosis by TPA in the epidermis of K5 E2F1 transgenic mice. Wild-type mice (A, C, and E) and K5 E2F1 line 1.1 mice (B, D, and F) were treated with 1.0 μg of TPA four times over 2 weeks. Skin sections were used for staining with hematoxylin and eosin (A and B), immunostaining for E2F1 protein (C and D), and performance of TUNEL assays (E and F). TUNEL-positive cells in panel F are marked by arrows.
