Figure 3.

Comparison of frequencies of CD24⁺CD27⁺IL-35⁺ (A) and CD24⁺CD27⁺TGF-ß⁺ (B) Breg of second-generation hepatitis B vaccine non-responders and responders. Depicted are (CpG+P/I)-induced frequencies of CD24⁺CD27⁺IL-35⁺ (A) and CD24⁺CD27⁺TGF-ß⁺ (B) Breg of second-generation hepatitis B vaccine non-responders (NR, red circles) and second-generation hepatitis B vaccine responders (R, blue circles) before (day 0) and 7, and 28 days after vaccination with a third- (NR group) or second- (R group) generation hepatitis B vaccine, respectively. The boxes show the median and the 25^th and 75^th percentile. Whiskers indicate the highest and lowest values. Statistical analyses were done with unpaired Mann-Whitney U tests.