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. 2021 Sep 10;12:746711. doi: 10.3389/fphar.2021.746711

FIGURE 1.

FIGURE 1

The cytotoxicity of Aβ1-42 in different aggregation stages in primary neurons. Aβ1-42 monomers were incubated by continuous orbital shaking at 150 rpm and 37°C. (A) The changes in the secondary structure of prepared Aβ1-42 in different aggregation stages were detected by circular dichroism (CD) spectra. (B) The proportions of the secondary structure were further analyzed. (C) The aggregation process of Aβ1-42 was investigated by the thioflavin T (ThT) assay (n = 4) and the cytotoxicity of Aβ1-42 in different aggregation stages in primary neurons was determined by the MTT assay (n = 5). (D) Representative transmission electron microscope image of Aβ1-42 incubated for 24 h at 37°C. Scale bars: 200 nm. Data are presented as the mean ± standard deviation (SD). *p < 0.05, **p < 0.01 and ***p < 0.001 compared with the proportions of β-sheets at 0 h (one-way ANOVA).