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. 2021 Sep 10;12:746711. doi: 10.3389/fphar.2021.746711

FIGURE 5.

FIGURE 5

Rhein inhibited mitochondrial oxidative stress-associated apoptosis in primary neurons induced by Aβ1-42 oligomers. Primary neurons were incubated with 5 μM Aβ1-42 oligomers and rhein at different doses (1, 5 and 10 μM) for 24 h at 37°C, respectively. Untreated primary neurons were the control group, and primary neurons treated with 5 μM Aβ1-42 oligomers alone were the Aβ1-42 group. (A) Representative images of Annexin V-FITC/propidium iodide (PI) double staining. Annexin V-FITC stains early apoptotic cells; PI stains late apoptotic cells. Scale bars: 50 μm. (B) Representative western blot images of cytosolic cytochrome c (cyto c). (C) Relative expression level of cytosolic Cyto c and normalization to β-Actin (n = 3). (D) Representative western blot images of cleaved caspase 3. (E) Relative expression level of cleaved caspase 3 and normalization to β-Actin (n = 3). Data are presented as the mean ± standard deviation (SD). **p < 0.01 and ***p < 0.001 compared with the Aβ1-42 group (one-way ANOVA).