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. 2021 Sep 10;12:746711. doi: 10.3389/fphar.2021.746711

FIGURE 7.

FIGURE 7

Rhein enhanced mitochondrial biogenesis by activating the SIRT1/PGC-1α pathway. Primary neurons were incubated with 5 μM Aβ1-42 oligomers and rhein at different doses (1, 5 and 10 μM) for 24 h at 37°C, respectively. Untreated primary neurons were the control group, and primary neurons treated with 5 μM Aβ1-42 oligomers alone were the Aβ1-42 group. (A) Representative western blot images of SIRT1. (B) Relative expression level of SIRT1 and normalization to β-Actin (n = 3). (C) Representative western blotting images of PGC-1α and NRF1. (D) Relative expression level of PGC-1α and normalization to β-Actin (n = 3). (E) Relative expression level of NRF1 and normalization to β-Actin (n = 3). Data are presented as the group mean ± standard deviation (SD). *p < 0.05, **p < 0.01 and ***p < 0.001 compared with the Aβ1-42 group (one-way ANOVA).