Table 3.
High touch environmental fomite samples.
| Sample type | Location |
CDC assay |
Spike assay |
|||
|---|---|---|---|---|---|---|
| N1 (CT) | N2 (CT) | RP (CT) | CT | TM | ||
| Door handle | Entry push button (Emergency Department) | UD | UD | UD | 38.2 | 72.5 |
| Exit push button (Emergency Department) | UD | UD | 39.0 | 36.9 | 76.1 | |
| Physician workroom (Emergency Department) | UD | UD | 36.1 | 37.8 | 80.2 | |
| Locker room (Emergency Department) | UD | UD | 37.3 | 37.1 | 74.6 | |
| Family consultation room (Emergency Department) | UD | UD | UD | 37.3 | 74.2 | |
| Resident workroom (Internal Medicine) | UD | UD | UD | 37.4 | 74.5 | |
| Nurses breakroom (Internal Medicine) | UD | UD | 40.8 | 36.8 | 75.8 | |
| Library workroom (Internal Medicine) | UD | UD | 37.8 | 36.0 | 75.2 | |
| Stairwell (Internal Medicine) | UD | UD | 36.9 | 36.0 | 75.2 | |
| Hospital Medicine office (Hospital Medicine) | UD | UD | 38.8 | 34.5 | 76.1 | |
| Other | Respiratory Care Center workstation(Emergency Department)a | UD | UD | 37.4 | 36.5 | 74.5 |
| Nursing workstation (Internal Medicine)a | UD | UD | 39.4 | 38.5 | 74.2 | |
| SARS- CoV-2 | 104 copies | 23.4 | 25.2 | – | 23.4 | 79.6 |
| 103 copies | 28.9 | 32.9 | – | 28.9 | 79.6 | |
| 102 copies | 32.8 | 36.9 | – | 33.7 | 79.1 | |
aIndicates samples collected from mouse and keyboards at shared workstations.
N1 and N2: CDC 2019-nCoV primer and probe mixes that target two viral nucleocapsid (N) genes for specific detection of SARS-CoV-2.
RP: primer and probe set that targets human RNase P gene; CT: cycle threshold; Tm: melting temperature;
UD: undetermined.