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. 2021 Sep 10;12:712948. doi: 10.3389/fimmu.2021.712948

Figure 2.

Figure 2

High salt diminishes cell activation and migration. (A, B) Flow cytometry analysis of surface expression of CD35 and CD66b on infected PMN 1 h post infection (means ± s.e.m.; n = 10; Student’s t test with Welch’s correction and Mann-Whitney test; *p < 0.05). (C) Extracellular bactericidal/permeability increasing protein (BPI) levels of control and infected (E. coli) PMN under NS or HS conditions (means ± s.e.m, n = 10-15; Mann-Whitney test; n.s., not significant; *p < 0.05). (D, E) As (A, B), but surface expression of CD11b and CD62L was analyzed (means ± s.e.m.; n = 10; Student’s t test with Welch’s correction and Mann-Whitney test; *p < 0.05). (F) Spontaneous movement of PMN [given in arbitrary units (a. u.)] upon E. coli-infection under NS or HS conditions. Scale bar: 50 µm (means ± s.e.m.; n = 40; each dot represents individual cell traces from two independent experiments; Mann-Whitney test; *p < 0.05). (G) Spontaneous and directed movement towards fMLP of PMN measured by Boyden chamber assays (means ± s.e.m.; n = 15-17; Student’s t test and Mann-Whitney test; n.s., not significant; *p < 0.05).